Coding

Part:BBa_K3929015:Design

Designed by: Carla Fabiola López Gómez   Group: iGEM21_UNIZAR   (2021-10-18)


Gene_Nb_CDR1_5773


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
    Illegal PstI site found at 29
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
    Illegal PstI site found at 29
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
    Illegal PstI site found at 29
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
    Illegal PstI site found at 29
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Before designing the three parts, sequence ends must be complementary in the correct order. Firstly CDR1, secondly CD2 and finally CDR3. Moreover, we found out that it recombines with higher efficacy than expected. Besides, none stability issues were shown.



Source

Its genetic sequence can be retrieved from Single Domain Antibody Database. Its specifications can be retrieved from Saerens, D.; Conrath, K.; Govaert, J.; Muyldermans, S. Disulfide Bond Introduction for General Stabilization of Immunoglobulin Heavy-Chain Variable Domains. Journal of Molecular Biology 2008, 377, 478-488.


References